Mastering Biology Homework Answers

Nucleic acids are assembled in the _____ direction.

The diagram below illustrates a lagging strand with the replication fork off-screen to the right.

Fragment A is the most recently synthesized Okazaki fragment.

The structure and orientation of the two strands are important to understanding DNA replication.

In DNA replication in bacteria, the enzyme DNA polymerase III (abbreviated DNA pol III) adds nucleotides to a template strand of DNA.

At each end of the replication bubble is a replication fork where the parental strands are unwound and new daughter strands (light blue) are synthesized.

Movement of the replication forks away from the origin expands the replication bubble until two identical chromosomes are ultimately produced.(Note that pol I stands for DNA polymerase I, and pol III stands for DNA polymerase III.) Even before the structure of DNA was elucidated, Erwin Chargaff and his coworkers noticed a pattern in the base composition of nucleotides from different organisms: the number of adenine (A) bases roughly equaled the number of thymine (T) bases, and the number of cytosine (C) bases roughly equaled the number of guanine (G) bases.Further, each species they studied had a different balance of A/T and C/G bases.We now know that these consistent ratios are due to complementary base pairing between A and T and between C and G in the DNA double helix, and interspecies differences are due to the unique sequences of bases along a DNA strand.In this exercise, you will apply Chargaff’s rules to predict the composition of nucleotide bases in a genome.Fragment B will be synthesized next in the space between primers A and B.Drag the labels to their appropriate locations in the flowchart below, indicating the sequence of events in the production of fragment B.In this activity, you will demonstrate your understanding of antiparallel elongation at the replication forks.Keep in mind that the two strands in a double helix are oriented in opposite directions, that is, they are antiparallel.Each of the four images below shows a strand of template DNA (dark blue) with an RNA primer (red) to which DNA pol III will add nucleotides.DNA replication always begins at an origin of replication.


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