Stem Cell Thesis

Stem Cell Thesis-39
Nestin was used as a marker to identify the cultured NSCs.Neuronal nuclei protein and glial fibrillary acidic protein (GFAP) were utilized to demonstrate the differentiation of NSCs towards neurons and astrocytes, respectively, in vitro.However, the in vivo ontogeny of VSCs remains controversial.

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The expression of all of the aforementioned proteins was detected using immunofluorescence methods.Alvetex® Strata has been previously shown to be a suitable substrate for the long-term maintenance of PSCs for enhanced differentiation (up to approximately 50 days).Here, we show that the same effect is possible from a single 10-day conditioning step, we term “priming”, with evidence of enhanced differentiation in vitro and in vivo.Neural stem cells (NSCs) are characterized by the ability of self‑renewal and capacity to proliferate and produce new nervous tissue.NSCs are capable of differentiating to three lineages of neural cells, including neurons, oligodendrocytes and astrocytes.The mechanisms of mechanotransduction in PSCs are poorly understood; the culture system developed here allowed for a direct comparison between two-dimensional (2D, conventional) and three-dimensional (3D) culture with substrate geometry as the only variable.The structure of the actin cytoskeleton, as well as that of intermediate filaments and microtubules, was less-developed after 3D culture.Cardiovascular diseases (CVD) are the leading cause of death in developed societies.Vascular stem cells (VSCs) have been proposed as a cell population with regenerative potential for CVD.This is in part likely to be a result of adaptation to the artificial culture environment, known to force cell structural away from the native state, resulting in altered gene expression.The mechanisms by which PSCs, specifically, integrate cues from their physical microenvironment remain largely unexplored.


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